coronavirus detection
Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.
Is temperature screening effective to detect the coronavirus disease?
Temperature screening alone, at exit or entry, is not an effective way to stop international spread, since infected individuals may be in incubation period, may not express apparent symptoms early on in the course of the disease, or may dissimulate fever through the use of antipyretics; in addition, such measures require substantial investments for what may bear little benefits. It is more effective to provide prevention recommendation messages to travellers and to collect health declarations at arrival, with travellers’ contact details, to allow for a proper risk assessment and a possible contact tracing of incoming travellers.
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
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Background The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and
Bovine coronavirus detection in adult cows in Brazil
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Winter dysentery is a bovine coronavirus (BCV)-caused disease that affects cows reported Europe, Japan, Canada and in the USA. The existence of enteric BCV infections in cows in an outbreak of diarrhea during the winter, a fact not reported in Brazil, has been surveyed in
Detection of SARS coronavirus in plasma by real-time RT-PCR
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to the editor: The identification and sequencing of a novel coronavirus1 associated with the recently described severe acute respiratory syndrome (SARS) 2 have permitted the development of antibody-based and genome-based tests for the infection. 4 Although
RNA-binding proteins of coronavirus MHV: detection of monomeric and multimeric N protein with an RNA overlay-protein blot assay
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The coronavirus mouse hepatitis virus (MHV, strain A59) consists of a helical nucleocapsid surrounded by an envelope containing two viral glycoproteins (Sturman and Holmes, 1977; Sturman et al, 1980). The nucleocapsid is composed of a 5.5 X lo6 Da positive-stranded
Detection of a murine coronavirus nonstructural protein encoded in a downstream open reading frame
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The coronaviruses are a group of RNA viruses with genomes of positive polarity which are about 27 kb in length (Boursnell et a/., 1987; Lai and Stohlman, 1978; Leibowitz et a/., 1981). Infected cells contain five or six subgenomic mRNAs, depending upon the virus studied, and
Detection of coronavirus in cases of tracheobronchitis in dogs: a retrospective study from 1971 to 2003.
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The reliance on postmortem material for this investigation likely underestimates the prevalence of infection with Canine coronavirus in cases of CITB for at least 3 reasons: 1) Similar to infection of humans with the closely related Coronavirus , OC4 an etiologic agent
Nested PCR assay for detection of bovine coronavirus S1 gene
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ABSTRACT A nested PCR targeted to the S1 ectodomain gene of the spike (S) glycoprotein of coronaviruses was developed to detect bovine coronavirus (BCoV) in stool specimens from cattle. One outer and one internal pair of primers were designed to conserved regions
Diagnostic detection of Wuhan coronavirus 2019 by real-time RT-PCR
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Background We used known SARS-and SARS-related coronaviruses (bat viruses from our own studies as well as literature sources) to generate a non-redundant alignment (excerpts shown in Annex). We designed candidate diagnostic RT-PCR assays before release of the
Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs
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Two methods of enzyme-linked immunosorbent assay (ELISA) were developed for the diagnosis of canine coronavirus (CCV) infection in dogs. One ELISA, in which CCV-infected CRFK cell lysate is used as antigen, is for the detection and titration of antibody against
Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system
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Facing the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), high-volume respiratory testing is demanded in laboratories worldwide. We evaluated the performance of a molecular assay for the detection of SARS-CoV-2 on a high-throughput
Detection of coronavirus disease (covid-19) based on deep features
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The detection of coronavirus (COVID-19) is now a critical task for the medical practitioner. The coronavirus spread so quickly between people and approaches 100,000 people worldwide. In this consequence, it is very much essential to identify the infected people so
An enzyme-linked immunosorbent assay using canine coronavirus -infected CRFK cells as antigen for detection of anti- coronavirus antibody in cat
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From the reasons that canine coronavirus (CCV) grows more efficiently than feline coronavirus in a cell culture and they are mutually related in their antigenicities, an enzyme- linked immunosorbent assay (ELISA) using CCV-infected feline kidney (CRFK) cells as
Detection of porcine respiratory coronavirus and transmissible gastroenteritis virus by an enzyme-linked immunosorbent assay
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An enzyme-linked immunosorbent assay (ELISA) for the detection of transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) was developed. A bovine TGEV-specific polyclonal antibody was purified by affinity chromatography with the
time amplification based methods-NASBA-Beacon, RT-PCR Taqman and RT-PCR hybridization probe assays-for the qualitative detection of SARS coronavirus
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The aim of this study was to develop a rapid, sensitive and robust procedure for the qualitative detection of SARS coronavirus RNA. Three unique detection formats were developed for realtime RNA amplification assays: a post amplification detection step with a
An ELISA for the detection of serum antibodies to both transmissible gastroenteritis virus and porcine respiratory coronavirus
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INTRODUCTION Transmissible gastroenteritis (TGE) is a disease of pigs caused by a coronavirus and characterized by a watery diarhoea often fatal to sucking piglets . In Britain, it has occurred as periodic epizootics, the last of which was in the winter of 1980/81. The virus also persist
The first detection of bovine coronavirus in calves diarrhea in west of Iran
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Background: Human T-cell lymphotropic virus (HTLV) infection is associated with adult T-cell lymphoma (ATL), tropical-spasticparaparesis (TSP) and HTLV-associated-myelopathy (HAM). In 2004 mandatory screening of corneal donors for HTLV was introduced. We
The indispensable role of chest CT in the detection of coronavirus disease 2019 (COVID-19).
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Dear Sir, We have read with interest the original article published online of the EJNMMI . The authors introduced the imaging and clinical characteristics of SARS-CoV-2 infected patients in Guangzhou, China. Notably, they reported the fact that a few patients who initially
A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics: SARS-CoV-2 DETECTR v3
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Given the global health emergency, rapid transmission, and severe respiratory disease associated with the outbreak of the 2019 novel coronavirus (SARS-CoV-2), Mammoth Biosciences has reconfigured our​ DETECTR platform to rapidly and accurately detect
Detection of human coronavirus NL63 in a 28 days old newborn in Iran
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1. Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. espiratory infections are one of the most frequent diseases with high morbidity and mortality in the first years of life. Human coronaviruses 229E, OC4 HKU1 and NL63
Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay
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An outbreak of novel betacoronavirus, SARS-CoV-2 (formerly named 2019-nCoV), began in Wuhan, China in December 2019 and the COVID-19 disease associated with infection has since spread rapidly to multiple countries. Here we report the development of SARS-CoV-2